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200104189's picture

Hi vrybody
I'm doing some IHC and I've been using poly-l-lysine to coat microscopic slides, now I'm out of poly-l-lysine and the waiting is killing me. I find out that I can use albumin/glycerol solution as an alternative.
Does anybody know the protocol for this?
Please guys help, it very urgent!

Ivan Delgado
Ivan Delgado's picture

Hi vrybody,
While you can coat slides for IHC with different compounds, coating them with albumin/glycerol is very different than coating them with poly-l-lysine. In other words you will likely get different results. What kind of detection are you trying to perform? Would using a different coating agent be an issue for this application?
Coating with albumin should be as simple as deciding on the amount to use (typically 1%), applying a layer over the slide, and then drying the slide. Pretty much the same way you would apply a layer of poly-l-lysine. You can always prepare a higher concentration solution to reduce the amount of time you incubate over the slide since albumin is a lot cheaper than poly-l-lysine.