Hydroxyproline assay

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ninakarla's picture
 Hello everyone,

 Hello everyone,

I need to do the hydroxyproline assay and so I followed the AOAC Official Method 990.26 (for meat and and meat products) but it didnt work even for standards (trans 4 hydroxy-L proline). I also tried the variations thereof but still I couldnt even make a standard curve. So I will greatly appreciate it if anyone will send me a protocol or method of the assay that surely works. Thank you!