Hydroxyproline assay

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Docmagus
Docmagus's picture
Hydroxyproline assay

Hello all.
I have some frozen hearts in my -80 and I wanted to analyize collagen content in them. I wanted to do a Hydroxyproline assay and was wondering if anyone had a good protocol.

My main questions are
1) Is the frozen tissue still good to do the assay with and how should it be handled?
2) What protocol is good for the assay?

Thanks all

cfish
cfish's picture
Here is the link to a

Here is the link to a protocol from our Protocol section.

http://www.scientistsolutions.com/index.php?a=protocol&c=10&p=391