how to neutralize my antibody

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tsukuba
tsukuba's picture
how to neutralize my antibody

hello every body

i identified one protein expressed in testis by using mass spectro, and created an antibody but i would like to certify if my antibody is good or not, because the western blot results of the total testis proteins gave me a single spot on the 2D gel but it looks lower than the MW of my protein. 
i kindly ask if some body knows the protocol for neutralizing the antibody using the antigen. 

thanks

g a
g a's picture
 Hi Tskuba

 Hi Tskuba

You can do an IP using your antibody followed with electrophoresis and identification of the protein by MS. That will directly tell you whether the Ab i specific to your protein or not?

tsukuba
tsukuba's picture
hello ARGERINE

hello ARGERINE

Thanks a lot.
 i actually don't want to use the MS again, my idea is, run two 2D gels one of them i use the antibody and for the other use the neutralized antibody, if the spot disappeared in the membrane treated with the neutralized antibody that will tell me my antibody is specific for my protein or not. right?
BUT i dont know how to neutralize my antibody.