How to find the problem in the signalling pathway?

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20101975
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How to find the problem in the signalling pathway?

How to find the problem in the signalling pathway? I want to know which techniques I should go for. Please be elaborative in your answers.

Shampa
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It depends which pathway you

It depends which pathway you are working on and also if you are giving any drugs as done for pre-clinical drug tests. Pls tell what kind of experiment you are performing.

20101975
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I am not giving any drugs.

I am not giving any drugs. its an experiment to find out the signalling pathway in the brain of the rat model of a disorder. I want to know if some changes are there in the brain of these rats or what. hence the experiment.