I'm using trying to get rat white fat samples to run properly on my gel. 120V, max amps is what I'm using now. My problem is that every second loaded sample is running into a funnel...but not all of them. Should I drop the voltage? Do you think there is protein aggregation (if so, what would you recommend?) My protein's around 31kDa. I started from scratch with the extractions, thinking something had happened there, but now these newly extracted samples are funneling more often than the last ones! (Still not every one though). Any ideas? By the way, I don't pour my own gels. I use the NuPage system.