I wanted to estimate the substrate Malonyl Co A ( of fatty acid biosynthesis) . What is the method for that ?
You need to do assay for Malonyl CoA. A highly sensitive high-throughput luminescence assay for malonyl-CoA decarboxylase is available at http://www.mitochondrial.net/showabstract.php?pmid=18294446&redirect=yes&terms=Malonyl+Co+A+assay
You can also download this pdf from here www.springerlink.com/index/LL251879206807H7.pdf . This would help you.