DNA extraction

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ilmare
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DNA extraction

Hi all,

I would like to do DNA extraction, but I 'm trying to avoid using phenol:chloroform. Does anyone a protocol for DNA extraction without using phenol or can suggest an alternative. Thanks in advance.

nin1318
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you can use any number of

you can use any number of kits. qiagen makes several good ones. they are inexpensive and simple to use.

genesan
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ilmare wrote:Hi all,

ilmare wrote:

Hi all,

I would like to do DNA extraction, but I 'm trying to avoid using phenol:chloroform. Does anyone a protocol for DNA extraction without using phenol or can suggest an alternative. Thanks in advance.

without phenol, if u want to do, u can replace it with isoamyl alcohol

ilmare
ilmare's picture
Hi Genesan,

Hi Genesan,

Thank you for your reply. So, you're saying that i can replace phenol:chloroform with just isoamyl alcohol alone?
Can you please tell how much isoamyl alcohol to use? If you have a protocol that you can give me, that would be great.