cryosectiong

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Judy Bohnert
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cryosectiong

Hello, I am using positively charged "Superfrost" slides to put my sections onto. I am sectioning ~20-40u sections of rat femur bone. The bone has been flash frozen and stored at -80, once coming out of 30%sucrose/Na Azide solution stored at 4 degrees . I put the bone into 30% sucrose and rock for 3 day-5 days then embedd in OCT for sectioning.Any suggestions as to why the sections are sometimes not sticking to the slides?Also sections are coming off during H&E staining/Masons Trichrome too.