Concanavalin A sepharose

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Emma147
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Concanavalin A sepharose

Help!!!!!!
How can I get my protein (myeloperoxidase) off my ConA column I've tried everything barr Chuck Norris

Guy Sovak
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Tell us what did you try? Guy

Tell us what did you try?
Guy

Emma147
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Ok,

Ok,
I have tried 0.5 M Methyl mannoside,
10 mM EDTA,
0.5 M Methyl mannoside pH 4.0
0.5 M methyl mannoside pH 2.0
4M Urea
20% Ethanol
all in sodium acetate buffer containing various amounts of NaCl, CaCl2, MnCl2 and MgCl2
Thank you:)