Clotting assay

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Amar Annamalai
Amar Annamalai's picture
Clotting assay

I need a best procedure to do clotting assay. I referred many articles to find it. At the end, I came to know I must have coagulometer to perform the clotting assay.

Kindly give me a procedure (universally accepted) that does not require coagulometer, if anyone knows.

Wishes
Amar

Guy Sovak
Guy Sovak's picture
Hi Amar,

Hi Amar,
What type of sample do you have,
Blood plasma?
Guy

Amar Annamalai
Amar Annamalai's picture
Yes. My sample is blood

Yes. My sample is blood plasma. I would like to do the clotting assay to measure tissue factor activity.

mvk
mvk's picture
Hi Amar,

Hi Amar,

You can do coagulation assay without coagulometer. You need water bath, accurate micropipette and foot operated stopwatch. If You refer old edition of "Practical Haematology" by Dacie & Lewis you will get the details.

Amar Annamalai
Amar Annamalai's picture
Thanks alot mvk. That work is

Thanks alot mvk. That work is still pending. I'll refer that text book and let you know if I have any questions. Thanks.