Chapters of Protocols

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pguo
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Chapters of Protocols

 Chapter 8. Immunohistochemistry / immunology
 
 
Preparing silanized (+plus) slides
Histological fixation
Microwave histology 
Microscopy Techniques
Confocal microscope technique

Electron microscopy
HE staining
DAPI & PI nucleic acid stain
Hybridization in situ
FISH
Special cell & cell fraction stains
Antibody purification
Antibody storage and handling
Conjugation of monoclonal antibodies
Antigen retrieval
Immunoperoxidase staining techniques
Immunofluorohistochemistry
Immunoprecipitation
Histotechnology--technical methods
FRQs for histochemistry
Laser Capture Microdissection
Elisa
Cytokine Assays Protocols  (1),  (2)
 
Chapter 9. Cellular Biology
 
General cell culture protocols
Tissue culture techniques
Chromosome karyotype
Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)
Cell cycle assay
Migration assay

Stem cell & related protocols

Apoptosis and related protocols
Soft Agar Assay for Colony Formation
Aorta ring assay
GFP transfection
Blood cell fractionation (white blood cell isolation)
Endothelial cell isolation and culture protocols
Flow cytometry (FCM)
 
Chapter 10. Metabolism, GC/MS, NMR and Proteomics
 
GC/MS Background
Glucose metabolism and its related protocols
Glucose and Insulin Tolerance Test
PAS staining
Deoxyribose procedure
Ribose metabolism analysis

Polysaccharide sequencing

Lactate cleanup and derivative
Amino acids
Fatty acids
Lipid protocols
Cholesterol
Bile acids
Urea procedure
Choline Incorporation Assay
Isotope Ratio Mass Spectrometer (IRMS)
Liquid chromatography / mass spectrometer (LC/MS)
Proteomics
NMR protocols and tutorials
 
Chapter 11. Animal Experiments
 
Blood sampling from animals
Basic skills for animal experiments
Anatomy and physiology of animals
Tail Vein Injection Techniques  (1)  (2)
Cancer xenograft animal models
Transgenic animal procedures
Transgenic cancer models
Murine models of human breast cancer
Animal models for depression-like and anxiety-like behavior
 
Chapter 12. Model Organisms 
 

C. Elegans protocols
Yeast protocols

 
Chapter 13. HPLC and TLC
 
HPLC protocols
TLC
 
Chapter 14. Buffers and Solutions in Lab.
 
Commonly Used Buffer and solution (114 formats in alphabeta)
Compound solutions (Practical Molecular Biology)
How to Make Simple Solutions and Dilutions (Department of Biology, Bates College)
Reto's Buffers&Media Book (Private Page)
Salt solutions (Practical Molecular Biology)
Single Component Solutions (Practical Molecular Biology)
Buffers (Gerard R. Lazo)
Antibiotics (Gerard R. Lazo)
Buffer Listing (Salmon Lab.)
Cell Culture Media and Solutions (Donis-Keller lab)
Commonly used solutions (1)--- 1 ) 2 ) 3 ) 4 ) 5 ) 6 )
Commonly used solutions (2)--- 1 ) 2 ) 3 )
Solutions for molecular cloning---1 ) 2 ) 3 ) 4 ) 5 ) 6 ) 7 ) 8 ) 9 )
 
Chapter 15. Other Resources
 
Free eBooks at Library Online
Super Textbook of Biochemistry
Cinema Online, Free Movies --(1) (2) (3)
Progresses in Life Science
Progress in Cancer Apoptosis -- (1) (2) (3)
Progress in Gene Therapy --- (1) (2) (3)
Progress in Neuronal Regeneration -- (1) (2) (3)
Progress in AIDS Treatment -- (1) (2) (3)
Progress in Organ Transplantation -- (1)  (2)  (3)
Progress in Stem Cell Research -- (1) (2)  (3)
Free eBooks in life science (1)
Free eBooks in life science (2,3)
Most Updated Biomedical Books
Pathway databases
Pathway Search
Biomedical Job Opportunities
Tools for Statistics
Free Software pDRAW32 to Draw DNA Analysis Charts
Biological Educational Resources
Genetics Education
Resources of Medical Biochemistry
Textbooks and Lab Manuals