i want to start immunocytochemistry for an enzyme in cultured cell please if sombody can help for providing protocol or link for that
Check the protocol section of the forum. There are a number of protocols for your application. Note that Enzyme and Protein are interconvertible for the IHC or ICC.
check following links as wellhttp://www.atlasantibodies.com/pdf/Immunohistochemistry_(IHC)_protocol.pdfhttp://www.bcm.edu/rosenlab/protocols/generalIHC.pdf
There are a number of companies which will provide a good source of antibodies for most applications such as Abcam or Cell Signalling. Which protein or enzyme you are looking for will tend to dictate your protocol.
yes i got that