cell culture

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Abhishek Singh
Abhishek Singh's picture
cell culture

i want to start immunocytochemistry for an enzyme in cultured cell please if sombody can help for providing protocol or link for that

Satinder
Satinder's picture
Check the protocol section of

Check the protocol section of the forum. There are a number of protocols for your application. Note that Enzyme and Protein are interconvertible for the IHC or ICC.
check following links as well
http://www.atlasantibodies.com/pdf/Immunohistochemistry_(IHC)_protocol.pdf
http://www.bcm.edu/rosenlab/protocols/generalIHC.pdf

heehawmcduff
heehawmcduff's picture
There are a number of

There are a number of companies which will provide a good source of antibodies for most applications such as Abcam or Cell Signalling.  Which protein or enzyme you are looking for will tend to dictate your protocol.
 

Abhishek Singh
Abhishek Singh's picture
yes i got that

yes i got that