catalase and sod assays

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200104189
200104189's picture
catalase and sod assays

Can somebody please help me with a catalase and sod assays(non-kit) based. I want to asses their activities on cancerous rat liver.
Thanx

hilltrekker
hilltrekker's picture
Hi, please see the below

Hi, please see the below pasted link. it shows modified technique for SOD
http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=2558
 
hope it will be of help

200104189
200104189's picture
Thanks very much Hilltrekker,

Thanks very much Hilltrekker, I will try it.
God bless

Satinder
Satinder's picture
 following articles may be

 following articles may be useful
doi:10.1016/S0009-8981(99)00246-6  
doi:10.1006/abio.1997.2273    
 

angelaeds
angelaeds's picture
Hi everyone,

Hi everyone,
i am in a very perplexed position. i have carried out the SOD assay using the NBT method. if im not wrong, the control solution (without plant sample) should give the darkest colour after 15 minutes of reaction time, while hte rest of the plant samples should be lighter, assuming the samples had some amount of SOD enzyme.
but instead, 2 of my plant samples became darker than the control solution. please help, i do not know what to do.

thanks
 

Abhishek Singh
Abhishek Singh's picture
is there direct

is there direct spectrophotometric assay for this

angelaeds
angelaeds's picture
Yes there is. You can find

Yes there is. You can find hte protocol as stated by hilltrekker although mine is a little different

Ruchi.s
Ruchi.s's picture
 @  angelaeds

 @  angelaeds
Same thing is happening with my assay.Did you come to know about its cause?