Hi everyone,
i am in a very perplexed position. i have carried out the SOD assay using the NBT method. if im not wrong, the control solution (without plant sample) should give the darkest colour after 15 minutes of reaction time, while hte rest of the plant samples should be lighter, assuming the samples had some amount of SOD enzyme.
but instead, 2 of my plant samples became darker than the control solution. please help, i do not know what to do.
Hi, please see the below pasted link. it shows modified technique for SOD
http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=2558
hope it will be of help
Thanks very much Hilltrekker, I will try it.
God bless
following articles may be useful
doi:10.1016/S0009-8981(99)00246-6
doi:10.1006/abio.1997.2273
Hi everyone,
i am in a very perplexed position. i have carried out the SOD assay using the NBT method. if im not wrong, the control solution (without plant sample) should give the darkest colour after 15 minutes of reaction time, while hte rest of the plant samples should be lighter, assuming the samples had some amount of SOD enzyme.
but instead, 2 of my plant samples became darker than the control solution. please help, i do not know what to do.
thanks
is there direct spectrophotometric assay for this
Yes there is. You can find hte protocol as stated by hilltrekker although mine is a little different
@ angelaeds
Same thing is happening with my assay.Did you come to know about its cause?