catalase and sod assays

8 posts / 0 new

12 May 2009

200104189

Can somebody please help me with a catalase and sod assays(non-kit) based. I want to asses their activities on cancerous rat liver.
Thanx

Tue, 05/12/2009 - 07:30

hilltrekker

Hi, please see the below

Hi, please see the below pasted link. it shows modified technique for SOD
http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=2558

hope it will be of help

Thu, 05/14/2009 - 07:57

200104189

Thanks very much Hilltrekker,

Thanks very much Hilltrekker, I will try it.
God bless

Thu, 05/14/2009 - 11:26

Satinder

following articles may be

following articles may be useful
doi:10.1016/S0009-8981(99)00246-6
doi:10.1006/abio.1997.2273

Tue, 08/04/2009 - 23:06

angelaeds

Hi everyone,

Hi everyone,
i am in a very perplexed position. i have carried out the SOD assay using the NBT method. if im not wrong, the control solution (without plant sample) should give the darkest colour after 15 minutes of reaction time, while hte rest of the plant samples should be lighter, assuming the samples had some amount of SOD enzyme.
but instead, 2 of my plant samples became darker than the control solution. please help, i do not know what to do.

thanks

Wed, 08/05/2009 - 02:28

Abhishek Singh

is there direct

is there direct spectrophotometric assay for this

Wed, 08/05/2009 - 20:47

angelaeds

Yes there is. You can find

Yes there is. You can find hte protocol as stated by hilltrekker although mine is a little different

Thu, 05/03/2012 - 10:20

Ruchi.s

@ angelaeds

@ angelaeds
Same thing is happening with my assay.Did you come to know about its cause?

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catalase and sod assays