Can somebody please help me with a catalase and sod assays(non-kit) based. I want to asses their activities on cancerous rat liver.
Hi, please see the below pasted link. it shows modified technique for SODhttp://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=2558
hope it will be of help
Thanks very much Hilltrekker, I will try it.
following articles may be usefuldoi:10.1016/S0009-8981(99)00246-6 doi:10.1006/abio.1997.2273
i am in a very perplexed position. i have carried out the SOD assay using the NBT method. if im not wrong, the control solution (without plant sample) should give the darkest colour after 15 minutes of reaction time, while hte rest of the plant samples should be lighter, assuming the samples had some amount of SOD enzyme.
but instead, 2 of my plant samples became darker than the control solution. please help, i do not know what to do.
is there direct spectrophotometric assay for this
Yes there is. You can find hte protocol as stated by hilltrekker although mine is a little different
Same thing is happening with my assay.Did you come to know about its cause?