biotinylation vs. immunoprecipitation

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Cynthia's picture
biotinylation vs. immunoprecipitation

Why would I choose to biotinylate rather than immunoprecipitate to determine protein-protein interactions? I am trying to determine if two proteins in one cell interact in a pathway. Which process would provide higher specificity?

qiang wang
qiang wang's picture
in your case, I personally

in your case, I personally prefer biotinylate. and would suggest chemical crosslinkage.

TigerShark's picture
I think it depends on how

I think it depends on how good your antibody is and hopefully not too expensive. With immunoprecipitation, you can often retrieve your natively folded protein/antigen for downstream functional studies. Sometimes with crosslinking methods, you are covalently modifying your protein which may cause concern that binding to other proteins is imparied.