I'm screening neutralizing antibodies by using B-cells, specially what's called ASC against a antigen on a microarray chip and phage-display.
This might be the issue about if the screened antibodies which are enable to bind the antigen during screening but I'm not sure if the bound one could be functional as neutralizing antibodies at the end.
What's the idea?
As one knowing almost nothing about the cencept - what's the neutralizing antibody?, I need your help to add your opinions to me.
As I know, there'd be some false-positives during selection or some existable mistakes during experiments, but beyond such exceptions, theoritically I want to ask you what the neutralizing antibodies are.
So, Is binding antibody all neutralizing antibody? Otherwise, what binds well could be just binding but possibly could be not always the neutralizing ones....
- Philipp Huh