ABTS antioxidant assay problem.....

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fatma_elzahraa
fatma_elzahraa's picture
ABTS antioxidant assay problem.....

Dear all,

I tried to measure the total antioxidant status in my serum samples... and the assay idea is based upon the bleaching of ABTS color with the more antioxidant in the sample...

really, i faced a crazy problem, the color increases with time.... i couldn't find a clue for this happenenig....
and the paper stated that the reagents are stable for 6 months...

and i made a clibration curve with trolox... and i got the best results in the curve... really i couldn't understand this problem...

i hope if there anyone can give a help....

Best regards