Quantifying immunofluorescence

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Sknman
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Quantifying immunofluorescence

Could someone tell me if it is worth quantifying antigens in paraffin sections using immunofluorescence? How many sections are needed, say with a heterogeneous antibody distribution such as in skin, and how do I safeguard that all unmasking the epitope in every sections would be uniform?If large numer of sections are needed, how to safeguard against quenching of fluorescence?

sknman