Monoclonal Antibodies: which PEG to use for fusion

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g a
g a's picture
Monoclonal Antibodies: which PEG to use for fusion

I have a confusion regarding which type of PEG shall be used for the fusion of murine myeloma cells with splenocytes......... Some of the protocols suggest the use of PEG 1500 and others suggest PEG 4000.
Sigma has both the grades and shows hybridoma tested.
Infact few people have even used PEG 8000. Please let me know as to which one is the best for cell fusion  and more importantly why?????
Warm regards
 

R Bishop
R Bishop's picture
Argerine,

Argerine,
 
PEG is a highly hydrated polymer that brings curved membranes together. It is hypothesized that somehow PEG causes disruption of the membrane packing that leads to a contraction of the two monolayers into one.  The mechanism by which this occurs is unknown oddly enough. The Lentz lab at the University of Nortyh Carolina is actively pursuing this topic though.  PEG 1500 is by far the most commonly used form for hybridomas.
 
Best
 
Rus

R Bishop
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Oh yeah here's a review and

Oh yeah here's a review and link to some more info
 
http://hekto.med.unc.edu:8080/FACULTY/LENTZ/fusion.htm

g a
g a's picture
Dear R Bishop

Dear R Bishop
Thank you for the post. I have recently purchased PEG 3700 mw from Sigma. Can you please further help for the precautions and disadvantages for using this instead of PEG 1500.
I will be starting cell fusion in coming week. It will be of  great help as I couldnt find much literature over the topic. Probably a first hand experienced person can guide me better.
Thanking you in anticipation
Regards