Immunoprecipitation Control

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Damani
Damani's picture
Immunoprecipitation Control

What is the best negative control for an IP? I am using IgG from the same species as the AB of interest. What do you do it it recognizes a band at the same MW as my protein of interest? Is there any better control for an IP of endogneous protein?

Guy Sovak
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In order to answer you in the

In order to answer you in the best way please let me know what are you treing to IP. Do you use a tag to pull down?
I use a non transfected cell line as a negative control. You can also use the same Non spesific IgG type that you are using for the IP.
Write me and I will help you more.
Guy

Damani
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Its not letting me email so I

Its not letting me email so I am posting the reply.
I am IP'ing endogneous nuclear protein our of cell lines and Tissue

Guy Sovak
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Thanks,

Thanks,
So use as a negative control non spesific IgG from the same sp. that your Ab is.
Let know if you had a background
I will ask hear a bit more about nuclear IPs
Guy