What do you look for in a secondary antibody?

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Deb1234
Deb1234's picture
What do you look for in a secondary antibody?

Hi Everyone,

Bit of a newcomer on here so I don't know if I've posted to the right place etc. but I would really appreciate help on writing an article about secondary antibodies. I've posted to this sub-forum because it is protocol related, as secondaries always have to be taken into account when developing any antibody based protocol.

It's simple really; all I ask you to do is post any helpful tips and advice you may have for both buying and using secondaries in any antibody based technologies you use, WB, ELISA, IHC, imaging, flow cytometry, etc. Even any interesting facts you may have about their characteristics and use!

A bit of a challenge I've taken on for one of my first projects (I'm writing an article for a newsletter about them), but I thought I'd give it a go!

Any help would be appreciated greatly and perhaps useful for forum users in future.

Thanks,

Deb1234

jyoti.sharma.s
jyoti.sharma.s's picture
Hi !

Hi !

Go through the following link for selection of secondary
antibodies -

http://www.jacksonimmuno.com/technical/select.asp

I hope this will be useful.

Deb1234
Deb1234's picture
Thank you!

Thank you!