scFv expression

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Sun Yi
Sun Yi's picture
scFv expression

I've selected the phage display antibody(scFv) library and transduced the phagemid into HB2151 for making scFv. But I can't get the scFv in several conditions, does any one know the optimal expression condition(especially the expression system,the concentration of glucose and IPTG, the tempreture,ect)which was determined empirically?
Thanks in advance!

Omai
Omai's picture
Here is a protocol that may

Here is a protocol that may help:

http://www.nature.com/nprot/journal/v2/n12/full/nprot.2007.455.html

Also, I'm going to move this question to the antibody forum to see if someone else can also help.

Omai

Sun Yi
Sun Yi's picture
Thank you very much!

Thank you very much!