Phalloidin and Methanol

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RachelD
RachelD's picture
Phalloidin and Methanol

Hello everybody,

I'm facing a well known problem with phalloidin: it doesn't work with a fixation in Methanol.
My issue is that the only antibodies i have found for my protein of interest and working in immunocytochemistery are working with the fixation in Methanol.
Somebody told me that i should try to start with a PAF fixation, and then 5 minutes with Methanol.
What do you think about this? I'm working on mouse primary cortical neurons.

Thank you very much,

Rachel