Optical immunoassay (OIA)

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lisazhang
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Optical immunoassay (OIA)

I am doing OIA and ELISA to detect snake venom for 3 months. Everytime I did OIA, I did ELISA in parallel. The conditons was optimized, and the results in ELISA were alwasy good. While the OIA cann't get good singal. After wash the chips there was almost What I use is SILAS chips. Ab were prepared by our lab, and I coat the chips and microplates by myself. I have changed different pH, Ab concentration, blocking, wash, dry..... Could someone give me some advice? Thanks.
lisa