IHC Counterstain - Nuclear Fast Red (Kernechtrot) Solution

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Tony Rook
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IHC Counterstain - Nuclear Fast Red (Kernechtrot) Solution

Please find the following media preparation method:

IHC Counterstain:

Nuclear Fast Red (Kernechtrot) Solution

Nuclear fast red ------------------------- 0.1 g

Aluminum sulfate ------------------------ 5 g

Distilled water ---------------------------100 ml

Dissolve aluminum sulfate in water. Add nuclear fast red
and slowly heat to boil and cool. Filter and add a grain of
thymol as a preservative.

Staining Pattern: Nuclear

Suggested Use:

Counterstain for immunohistochemistry or other special staining.

Suggested Staining Time:

5 minutes

Link Reference:
http://www.ihcworld.com/_protocols/counterstain_solutions/nuclear_fast_red.htm