Gill's Hematoxylin Solution

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Tony Rook
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Gill's Hematoxylin Solution

Please find the following media preparation method:

Gill's Hematoxylin Solution

Chemicals Single Double Triple Function
Ethylene glycol 250 ml 250 ml 250 ml solvent
Distilled water 750 ml 750 ml 750 ml solvent
Hematoxylin 2 g 4 g 6 g dye
Sodium iodate 0.2 g 0.4 g 0.6 g oxidizing agent
Aluminum sulphate 20 g 40 g 80 g mordant
Glacial acetic acid 20 ml 20 ml 20 ml pH control

Method:

Dissolve ethylene glycol in distilled water.

Add hematoxylin, then sodium iodate.

Add aluminum sulphate and acetic acid

Stir for 1 hour at room temperature.

Filter if necessary.

Staining Characteristic - Progressive

Suggested Use

Single strength for cytology.

Triple strength for immunohistochemistry counterstain (dilute 1:5) for 30 seconds and H&E (dilute 1:5) for 5 minutes.

Note

This recipe gives clear and sharp nucleus staining with little background. Good for both immunohistochemistry counterstain and H&E. This solution is also alcohol and mercury free so it is good for IHC counterstain when AEC substrate is used. When triple strength is used, dilute 1:5 with distilled water before use.

Link Reference:
http://www.ihcworld.com/_protocols/counterstain_solutions/gill_hematoxylin.htm