Please find the following media preparation method:
Gill's Hematoxylin Solution
Chemicals Single Double Triple Function
Ethylene glycol 250 ml 250 ml 250 ml solvent
Distilled water 750 ml 750 ml 750 ml solvent
Hematoxylin 2 g 4 g 6 g dye
Sodium iodate 0.2 g 0.4 g 0.6 g oxidizing agent
Aluminum sulphate 20 g 40 g 80 g mordant
Glacial acetic acid 20 ml 20 ml 20 ml pH control
Method:
Dissolve ethylene glycol in distilled water.
Add hematoxylin, then sodium iodate.
Add aluminum sulphate and acetic acid
Stir for 1 hour at room temperature.
Filter if necessary.
Staining Characteristic - Progressive
Suggested Use
Single strength for cytology.
Triple strength for immunohistochemistry counterstain (dilute 1:5) for 30 seconds and H&E (dilute 1:5) for 5 minutes.
Note
This recipe gives clear and sharp nucleus staining with little background. Good for both immunohistochemistry counterstain and H&E. This solution is also alcohol and mercury free so it is good for IHC counterstain when AEC substrate is used. When triple strength is used, dilute 1:5 with distilled water before use.
Link Reference:
http://www.ihcworld.com/_protocols/counterstain_solutions/gill_hematoxylin.htm