Vote on best antibody for FLAG tag

1 post / 0 new
ZhanglinShen's picture
Vote on best antibody for FLAG tag

 Hello everyone,

I would like to start a discussion/vote on which antibody (polyclonal or monoclonal) works best for detecting FLAG fusion protein in Western blot.

I have used the M2 from Sigma|BRAND_KEY&N4=A2220|SIGMA&N25=0&QS=ON&F=SPEC

and also the rabbit anti ECS from Dualsystems Biotech:

Both antibodies detect my fusion proteins (some of which are up to 250 kDa). The M2 is a bit cleaner,  but seems to be less sensitive (?). Also, the M2 is quite expensive and I wonder if there are any other options out there?

Thanks for your ideas!