I would like to start a discussion/vote on which antibody (polyclonal or monoclonal) works best for detecting FLAG fusion protein in Western blot.
I have used the M2 from Sigma
and also the rabbit anti ECS from Dualsystems Biotech: http://www.dualsystems.com/products/antibodies.html
Both antibodies detect my fusion proteins (some of which are up to 250 kDa). The M2 is a bit cleaner, but seems to be less sensitive (?). Also, the M2 is quite expensive and I wonder if there are any other options out there?
Thanks for your ideas!