I am getting lots of problems with anti HSP90.
Would like to get some idea of antibody that you are using.
I am using Stressgen , its not working.
I found a solution,
So I am using an Anti Rabbit anti HSP90 Ab.
In my case I incubated it over night.
This antibody is reactive with both the constitutive and the inducible forms of HSP90. It does not bind to the native form.
HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress. Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP- and co-chaperone-dependent manner. Polyclonal antibodies are produced by immunizing animals with synthetic peptides surrounding Glu289 of human HSP90. Antibodies are purified by protein A and peptide affinity chromatography. HSP90 Antibody detects endogenous levels of total HSP90 protein. This antibody does not cross-react with other HSPs. Munohistochemical analysis of paraffin-embedded human colon carcinoma, using HSP90 (E289) Antibody. Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HSP90 (E289) Antibody in the presence of control peptide (left) or antigen-specific peptide (right). Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using HSP90 (E289) Antibody.
Thanks and Regards,
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