Dynein subunit knockdowns reveal their role in otolith formation

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Jon Moulton
Jon Moulton's picture
Dynein subunit knockdowns reveal their role in otolith formation

Otoliths, small mineralized structures in the inner ear, are formed by a process which requires the activity of cilia. Morpholino knockdowns of dynein-related proteins inhibit otolith formation.

Colantonio JR, Vermot J, Wu D, Langenbacher AD, Fraser S, Chen JN, Hill KL. The dynein regulatory complex is required for ciliary motility and otolith biogenesis in the inner ear. Nature. 2008 Nov 30. [Epub ahead of print]

http://www.ncbi.nlm.nih.gov/pubmed/19043402

Guy Sovak
Guy Sovak's picture
That is an interesting paper.

That is an interesting paper.
For those whom would like some additional information about Otoliths click on this link:
http://www.tchain.com/otoneurology/disorders/bppv/otoliths.html
Guy

Jon Moulton
Jon Moulton's picture
Well Guy, your post piqued my

Well Guy, your post piqued my curiosity about otolith work with Morpholinos. Recalling a few papers, I searched the literature for otolith papers using Morpholinos and found a more extensive body of work than I had expected.

Blasiole B, Canfield VA, Vollrath MA, Huss D, Mohideen MA, Dickman JD, Cheng KC, Fekete DM, Levenson R. Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish. Dev Biol. 2006 Jun 1;294(1):148-60. Epub 2006 Mar 29

Hardison AL, Lichten L, Banerjee-Basu S, Becker TS, Burgess SM. The zebrafish gene claudinj is essential for normal ear function and important for the formation of the otoliths. Mech Dev. 2005 Jul;122(7-8):949-58.

Hughes I, Blasiole B, Huss D, Warchol ME, Rath NP, Hurle B, Ignatova E, David Dickman J, Thalmann R, Levenson R, Ornitz DM. Otopetrin 1 is required for otolith formation in the zebrafish Danio rerio. Dev Biol. 2004 Dec 15;276(2):391-402.

Kang YJ, Stevenson AK, Yau PM, Kollmar R. Sparc Protein Is Required for Normal Growth of Zebrafish Otoliths. J Assoc Res Otolaryngol. 2008 Sep 11. [Epub ahead of print]

Murayama E, Herbomel P, Kawakami A, Takeda H, Nagasawa H. Otolith matrix proteins OMP-1 and Otolin-1 are necessary for normal otolith growth and their correct anchoring onto the sensory maculae. Mech Dev. 2005 Jun;122(6):791-803.

Petko JA, Millimaki BB, Canfield VA, Riley BB, Levenson R. Otoc1: A novel otoconin-90 ortholog required for otolith mineralization in zebrafish. Dev Neurobiol. 2008 Feb 1;68(2):209-22.

Sollner C, Burghammer M, Busch-Nentwich E, Berger J, Schwarz H, Riekel C, Nicolson T. Control of crystal size and lattice formation by starmaker in otolith biomineralization. Science. 2003 Oct 10;302(5643):282-6.

Sumanas S, Larson JD, Miller Bever M. Zebrafish chaperone protein GP96 is required for otolith formation during ear development. Dev Biol. 2003 Sep 15;261(2):443-55.

And to reiterate the citation that started this thread:
Colantonio JR, Vermot J, Wu D, Langenbacher AD, Fraser S, Chen JN, Hill KL. The dynein regulatory complex is required for ciliary motility and otolith biogenesis in the inner ear. Nature. 2008 Nov 30. [Epub ahead of print]