i.v. injection of human recombinant TNF-alpha

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ltrbrown
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i.v. injection of human recombinant TNF-alpha

I am hoping that someone may have some experience with i.v. injection of human recombinant TNF-alpha. I am going to test the effects of this protein on the growth potential cell lines in Nude mice. My research of the literature shows doses that range from 4 ug to 10 ug ("max tolerated dose") per mouse, by i.v. injection. Could anyone give advice regarding these numbers, and suggest symptoms that indicate toxicity (weight loss and others?). Most people did a single injection and followed tumor growth for up to 12 days, which (I think) indicates that TNF-alpha is pretty stable. My Supervisor would like me to dose at 8ug per mouse per week for 3 weeks, but I feel this may be excessive.