how to analyse the intravaginal calcium,potasium and chloride in cyclic buffalo ???
if you have access to the below mentioned paper i hope it will be of some help to youConcentration of calcium, zinc, magnesium, potassium and sodium in the bovine cervical mucus during normal oestrus and oestrus induced by progesterone and/or PGF2α
T. Tsiligianni, A. Karagiannidis, N. Roubies, P. Saratsis and P. Brikas
Ninety-two healthy Friesian cows were used to determine the concentration of calcium (Ca), zinc (Zn), magnesium (Mg), potassium (K) and sodium (Na) in bovine cervical mucus and to compare these concentrations in cervical mucus collected during spontaneous and induced oestrus. Cows were assigned to four groups: (1) no treatment (2) PRID for 12 days plus pregnant mare's serum gonadotrophin (PMSG; 1000 IU, i.m.) at the removal of the PRID (3) PGF2α , two doses (6 mg, i.m.) 11 days apart, and (4) PRID for 7 days plus one dose of PGF2α (6 mg, i.m.) 24 h before removal of the PRID. The cows of the three induced-oestrus groups received two artificial inseminations (AI), whereas those in the spontaneous-oestrus group received only one. Cervical mucus samples were collected from all cows 5 to 30 min before the first AI. The results of this study can be summarized as follows: (1) viscosity was significantly lower (P<0.001) and crystallization was significantly (P<0.001) higher in the cervical mucus of cows in the spontaneous-oestrus group compared with cows in the induced-oestrus groups; (2) Na concentration was similar in all groups; (3) concentration of all the other elements in the spontaneous-oestrus group was significantly lower (P<0.05) than in the induced-oestrus groups; (4) there was significant positive correlation (P<0.001) between viscosity and all elements, and significant negative correlation (P<0.05–P<0.001) between crystallization and all elements.Reproduction, Fertility and Development 14(7) 427 - 431
here is other link not related to your querry but just for your referenceBUFFALO PRODUCTION AND RESEARCHftp.fao.org/docrep/fao/010/ah847e/ah847e.pdf
Great pundir !! this may help in my research also.
Thank u sir iam already having one related article but thats not enjoug for me but this article very close my expectation so i will refer this paper