Thioglycolate and pain management in mice

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inthebluelab
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Thioglycolate and pain management in mice

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I'm going to be extracting macrophages from mice though intraperitoneal lavage, having recruited the macrophages to the site through injection of thioglycolate to into the IP four days prior to culling. I have plenty of info on that protocol, and the preparation of thioglycolate. What I don't have is any information on pain management or care of the mice after the injection of thioglycolate.

Does anyone have any information or advice for caring for mice with induce inflammatory responses? I need to amend my ethics approval if I’m injecting the mice while they’re alive, and I also don’t want to risk my mice dying or being in pain that could be relieved.

Kermit
Kermit's picture
Hi!

Hi!
I am currently in the same situation as you, needing to amend my ethics to perform this protocol and I am looking for pain and how the mice handle the injection...
Did you have any information since then??
Thanks for your help!

victorius
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From a IACUC protocol found online:
Thioglycollate injections i.p.: We have over two years of experience with thioglycollate elicited macrophages.  The injected mice do not exhibit more than momentary discomfort or pain, primarily immediately after injection.  The mice do not exhibit sickness behavior of any type during the post-injection period.  However, in view of veterinarian concerns, we will monitor injected animals for any signs of sickness behaviors and if clear signs indicative of a generalized disease process (infection) or signs of distress become visible after thioglycollate administration, mice would be immediately euthanatized.

In our protocol we list TG injections as Class E pain, but monitor animals for pain/discomfort and sac' them if they show it.

Regards,

V.

Mary Bailey
Mary Bailey's picture
I am currently in the same situation

I am currently in the same situation as you, needing to amend my ethics to perform this protocol and I am looking for pain and how the mice handle the injection. (soundcloud to mp3 & kissmanga)