i am trying to prepare 8% casein in pbs for peritonial recrutment of neutrophils but i am facing problem in dissolving casein in pbs.
kindly suggest me the posible pitfalls as i am doing it for first time.
As you rihghtly not it is very difficult to prepare 8% casin solution,
For that you need to have bit patience, you add step by step and vortex properly intialy you take RT PBS and after some time you incubate at 37oC for some time and again vortex it properly
kindly suggest me what is RT PBS and i shall be thankfull to you for explaining the process in detail as i am inexperinced in this matter.
just tell me the way you dissolve it as all protocol tell it to incubate at 37 or 70 *C with megnatic stirer.
i am trying to dissolve it by periodically adding small amount of casein to hot pbs (50-70*C on magnetic stirrer) but after autoclaving all casein converts to insoluble pink clump.