electrophoresis

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kannappan
kannappan's picture
electrophoresis

could anyone suggest me an alternative tank buffer other than tris-glycine running buffer along with its composition.

Ivan Delgado
Ivan Delgado's picture
Hi Kannapan,

Hi Kannapan,
You can try Tris-HEPES. A complete protocol, including all solutions, can be found here.
Another type of gel is Tris-Tricine. Here is a protocol.
Finally, there is bis-Tris. Here is a complete protocol.
Cheers

msvnathan
msvnathan's picture
hi

hi
can u tell little detaily why u want to change the buffer and what kind of problem u r facing?