I am patching mammalin cell lines. Recently I found that when the pipette touches the cell, the graph of seal test wobbles greatly, I mean not the number of resistance wobble,which was change little, just the graph especially the part of flat roof wobble most greatly. I did not give cell much pressure and my pipette is not very thin. why this happen?
please help me
wobble of seal test
2 June 2009
#1
zhangxu04
wobble of seal test
Dear Zhangxu04
A wobbling baseline during a seal test is a sure sign that you have advanced the pipette too far and you are actually pressing down through the cell onto the base of the coverslip.
Are you patching "thin" cells like CHO K1's?
thank you very much I used the h293A
PM : I will do some hERG assay with patch clamp. could you recommamd some practical paper for me ? thank you very much.