Whole-Cell Patch Clamp - Electrode in the bath current response.

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abadehar
abadehar's picture
Whole-Cell Patch Clamp - Electrode in the bath current response.

 Hello, 

I am trying to perform a whole-cell patch clamp on Min6 cells. Recently I am getting a different current response when my micropipette enters the bath solution and before it makes any contact with a cell. I have attached the current response I used to see when everything was working fine.

I have recetly learned the technique so I am not quite sure what might cause this to happen. 

Thank you for your time.