Which membrane-rupturing method is better for avoiding reseal

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engstar
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Which membrane-rupturing method is better for avoiding reseal

Which method between zapping and suction is better in terms of avoiding reseal of the membrane in whole cell recording?
Thanks.  

cherian.lijo
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This portion of going into

This portion of going into whole cell config from seal attached is an art. It takes time to get your hands trained for it. You can use initially a small syringe suction and followed by low volt zap this will also keep the seal intact. You can also try using mouth suction for more controlled suction.