What should I do to use sylgard ?

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Recorder
Recorder's picture
What should I do to use sylgard ?

Hello,

I'm trying to do cell attached patch, and I want to use sylgard to reduce capacitance.

I read about using sylgard many times here and there, but still I don't know exactly how I can use it.

Do they actually mean that I have to paint sylgard with something like brush? close to the tip of the pipette? under the microscope?

Or can I just immerse it to sylgard solution?

Anyone who can let me know about it in detail, please?

Thank you.

Lostbrain
Lostbrain's picture
 Sylgard is like a type of

 Sylgard is like a type of glue. If you submerge the pipette tip you will obviously block it.
In order to get close to the tip with sylgard (you need that to get rid of glass capacitance affecting your recording), you will need to see the tip. For that you need a microscope adapted to this purpose. You can probably do it with just a good magnifier and something to hold the pipette on focus. You can use a brush or simply a plastic pipette tip to get some sylgard. Then, carefully you will have to touch with the sylgard the pipette, the closest to can to the tip. Then extend the sylgard towads the other end. After that you will need to heat the sylgard (it gets solid in this way). For that you need a dryer (like the one for hear) but be careful because the sylgard will move with the air (put the dryer in hot mode but low flow, if you can). Follow your instinct ;)