use of aspartate, glutamate or gluconate in the intracellular solution

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uppies
uppies's picture
use of aspartate, glutamate or gluconate in the intracellular solution

can someone provide me the reason for substituting either Asp, Glu, or gluconate in the intracellular solution for chloride? I have noticed that for TRPM2 recordings, no one uses any chloride in their intracellular, and i am confused as to why this is.
 

neuro
neuro's picture
ions like gluconate,

ions like gluconate, methanesulfonate, or aspartate (i've never heard of glutamate being used) are larger than chloride, with lower mobilities.  hence they contribute less to the liquid junction potential.  you are correcting for your LJP aren't you?

uppies
uppies's picture
yeah, i am. I think the issue

yeah, i am. I think the issue is primarily with potentially opening up chloride channels, particularly in oocytes, as there are Ca2+ activated chloride channels.