I am new to patch clamping and wantd to ask about whole cell capacitance and series resistance.
One I break into my cell, I clamp and -60mV and input a pulse of -4mV. I then put on wholce cell capacitance to reduce the fast component of my transients (the slow components remain). Once I have done this I apply series resistance. I normally just put on a bit of both until the fast transient component is gone (is this correct?). I then finally put on compensation. I am using an axopatch 200B amplifier.
Q1 Why do I want to cancel the fast part of the transients? Is it beause it gives me a rough measurement of the cell size? Is there any other reason? Do some people cancel all of the transients? Do some people not?
Q2 How to I calculate how much compensation to put on? I have been told different things by different members of my lab.
Any help would be greatly appreciated!!!