TRP channel isolation

1 post / 0 new
slimlyn08
slimlyn08's picture
TRP channel isolation

Hi!

I'm trying to purify 5 TRP channels (TRPC-1, TRPV-1, TRPM-7, TRPA-1, and TRPN-1, buy don't know where to start!
How can I purify the individual channels, and put them into liposomes?

After I put them into liposomes, how can I test them using patch clamp assay??