I have observed a developmental reduction in Kv current in a particular set of coincidence detecting neurons. It is widely believed that these neurons also show a developmental increase in leak current. I dont want to argue about whether I have a pure H&H leak or some moderately rectifying leak and I know many of you want more details but I think this is a conceptual question.
As these cells age, the membrane seems more difficult to access further compounded by the reduced visibility due to myelination. The two explanations for the reduction in Kv current over development is a) I did not have a gigahom seal or b) a strong K-leak current offset the outward Kv current.
I observe up to 30nA of current (at 40mV) so I imagine a large amount of K leaving, and because the amount is so large I see some of K ions remaining around the recorded neuron, locally changing the driving force for these ions. If K-2pore leak channels were present, would the K ion influx in enough quanity as to offset a substainal portion of outward Kv current?