Dear Patch Clampers,
I'm completely new in this field.
I would like to know if someone is using Sigmacote to coat their micropipettes instead of sylgard? Is it better?
Would you please explain to me how to do it, do I need to dip it in the Sigmacote after filling it with the internal solution? Do I need to keep a positive pressure? How long do I have to wait.
Many thanks in advance.