thanks for your attention!
I'd like to know the the membrane resistance of the "opened" HEK293 T cells.
before "open" the cell, My Seal Resistance can up to several G ohm, and at the beginning of "Zap", the Rm dereases to about 100 M ohm,one or two minutes later the Rm rise to several hundreds.
This is my first time to use the HEK293 cells, can anybody tell me are the cells healthy? or what's your membrane resistance after open the cell?
Rmembrane of the HEK293