I have question on patching at physiological temperature (35-37C)?
Should I do patch at room temerature first, and then the heating system should be on? I mean, the following 1-3 steps should be applied to each cell? Or can I keep the heating system on always (from the beginning the day until the end)?
1: Achieve whole cell configuration at room temperature
2: Bring the temeprature to the physiological level
3: Start to do experiment