The best way I have found to avoid resealing is to spend time designing a new program on the puller that pulls wider tips that still have the desired resistance when filled with intracellular solution. How wide are you tips? See if you make them at least 1.5 microns inner diameter at the tip.
Another trick if you have not already done so is to increase the EGTA concentration to 10mM in your pipette solution.
The best way I have found to avoid resealing is to spend time designing a new program on the puller that pulls wider tips that still have the desired resistance when filled with intracellular solution. How wide are you tips? See if you make them at least 1.5 microns inner diameter at the tip.
Another trick if you have not already done so is to increase the EGTA concentration to 10mM in your pipette solution.