We did inside-out and cell-attached rcording.
1. the refferentce electrode: Ag-AgCl wire inserted into agar containing 3MKCl.
2. the bath and pipette solutions are the same, containing (in mM) : potassium aspartate 140, EGTA 5, MgCl2 2 and Hepes 10, and pH adjusted to 7.4.
3. Smooth myocyte.
4. Amplifier:Pclamp 200A
1. May the junction potential at the interface berween Ag-AgCl wire and pipette solution change and influence clamp potential? Is that caused by the change of Cl- activity in the pipette solution during recording?
2. We thought that becouse the single channel current is small and recorded in short time the change of the junction potential would be small and influence little. Is that right?
3. How to estimate the change of the junction potential at this inteface quantitively?
4. How to correct this junction potential?
Thanks every one