I am interested in trying perforated patch.
1: using amphotericin B, how long does it take normally to arrive at acceptable whole cell mode? I have used nystatin and I remeber it took several min.
2: Should I fill up amphotericin B in the electrode tip first, and then back fill the intra solution?
3: Specific tips for handling amphotericin B. Stored frozen and once thawed, should it be used in a short (some min)?