Measurement of Membrane Capacitance

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cherian.lijo
cherian.lijo's picture
Measurement of Membrane Capacitance

Hi,

You guys forum and suggestions are really wonderfull!!!!

I am working with Axopatch 1D, Clampex-9 on HEK293T cell lines, recently we had problem of incorrect capacitance displayed in the software, now with the technical assistance from axon that problem is solved. But I would like to calculate capacitance experimentally, So can anyone please suggest a good reference describing the protocol and a simple method followed to calculate Memebrane capacitance of a cell.

Thank you,

Abhishek Singh
Abhishek Singh's picture
please go through coultor

please go through coultor counter the measure resistance

cherian.lijo
cherian.lijo's picture
 But  I am looking for some

 But  I am looking for some protocol in voltage clamp or current clamp mode to be used with my setup to measure the capacitance

lazy
lazy's picture
Usually I use the value of C

Usually I use the value of C-slow as membrane capacitance.

If you like to obtain the experimentally measured value of capacitance, I would record an electrotonic potential in order to obtain the value of taw. From that, I would be able to obtain the value of the membrane capacitance. The following is the method.

With current clamp mode, I would pass a small (negaive) current, and you would obtain the electrotonic potential (from the data, you can measure the membrane resistance and taw).

From the equation of:
taw = R x C

You would obtain the value of C with your experimental data. This should be the valueof the capacitance of your cell.
Hope this helpful.

cherian.lijo
cherian.lijo's picture
Thank you!!!

Thank you!!!