Hello! For my diploma thesis I am trying to photoinactivate neurons in the cricket's auditory system. Therefor I have to fill the neuron iontophoretically with lucifer yellow. Unfortunately, lucifer yellow blocks my micropipettes. I'm using 5% LuYe in 0.5m Lithiumchloride and 1m Lithiumchloride for backfilling the pipettes. If I fill the pipettes just with LiCl, I get nice intracellular recordings, but with LuYe they block almost instantly. Do you have any tips or ideas?